Fig. 6

lnc-MLETA1 promotes cell motility through the miR-186-5p-EGFR and miR-497-5p-IGF1R axis. A RNA-sequencing of total RNA extracted from lnc-MLETA1-knockdown and control CL1-5 cells are presented in a heatmap. B Gene Set Enrichment Analysis (GSEA) of published metastasis gene signatures in lnc-MLETA1-knockdown cells versus control cells. C GSEA of miRNA target gene signatures in lnc-MLETA1-knockdown cells versus control cells. D Left: venn diagram of intersection of predicted miR-186-5p target gene and downregulated gene after silencing lnc-MLETA1. Right: venn diagram of intersection of predicted miR-497-5p target gene and downregulated gene after silencing lnc-MLETA1. E qRT-PCR and F Western blot analyses of EGFR expression of lnc-MLETA1-knockdown and control CL1-5 cells. G qRT-PCR and H Western blot analyses of IGF1R expression of lnc-MLETA1-knockdown and control CL1-5 cells. I qRT-PCR and J Western blot analyses of EGFR expression of miR-186-5p-overexpressing and control CL1-5 cells. K qRT-PCR and L Western blot analyses of IGF1R expression of miR-497-5p-overexpressing and control CL1-5 cells. M Luciferase activity of CL1-0 cells transfected with miR-186-5p mimics and pMIR-REPORT luciferase plasmid which contain 3'UTR of EGFR. Data are presented as the ratio of Renilla luciferase activity to Firefly luciferase activity. N Luciferase activity of CL1-0 cells transfected with miR-497-5p mimics and pMIR-REPORT luciferase plasmid which contain 3'UTR of IGF1R. O Western blot analysis of EGFR expression of CL1-5 cells co-transfected with lnc-MLETA1 plasmids or control plasmids and with miR-186-5p mimics or negative control. P Western blot analysis of IGF1R expression of CL1-5 cells co-transfected with lnc-MLETA1 plasmids or control plasmids and with miR-497-5p mimics or negative control. Q Left: representative images of wound-healing assay of CL1-0 cells co-transfected with lnc-MLETA1 plasmids or control plasmids and with shEGFR, shIGF1R, or control shScramble. Scale bar, 200 μm. Right: the number of migrated cells was counted. Results are presented as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Two-tailed Student’s t-test