Fig. 1

FAM171B expression is positively correlated with tumor progression and M2 TAM infiltration in BLCA patients. A Analysis of FAM171B expression in tumor tissue and paired normal tissue from TCGA dataset. B Representative IHC staining images of FAM171B in BLCA tissue array. C IHC score in different T stages from BLCA tissue array. D IHC score in different N stages from BLCA tissue array. E Relationship between FAM171B expression level and overall survival from TCGA dataset. F Relationship between FAM171B expression level and progression free survival from TCGA dataset. G Relationship between FAM171B expression level and disease specific survival from TCGA dataset. H Relationship between FAM171B expression level and disease-free survival from TCGA dataset. I Relationship between FAM171B expression level and overall survival from GEO dataset (GSE13507/GSE31684/GSE32894/GSE37815). J Analysis of FAM171B expression in different T stages from TCGA dataset. K Analysis of FAM171B expression in different N stages from TCGA dataset. L Analysis of FAM171B expression in different pathologic stages from TCGA dataset. M Lollipop plot demonstrating the correlation between FAM171B expression and different immune cell infiltration. N Heat map of the correlation between FAM171B expression and major monocyte/macrophage chemokines. O Immunoscore analysis of FAM171B and M1 macrophages in bladder cancer using QUANTISEQ algorithm. P Immunoscore analysis of FAM171B and M2 macrophages in bladder cancer using QUANTISEQ algorithm. Q Representative IHC staining images (FAM171B) and IF staining images (CD206, CD8 and α-SMA) in BLCA tissue array. R Proportion of immunofluorescence-positive cells (CD206, CD8 and α-SMA) in FAM171B high or low expression group from tissue array