Fig. 2

FAM171B promotes the malignant phenotype of bladder cancer cells. A The Chronos dependency score analysis of FAM171B in the 28 human bladder urothelial carcinoma cell lines in the CCLE database. The Chronos dependency score is based on data from a cell depletion assay. A lower Chronos score indicates a higher likelihood that the gene of interest is essential in a given cell line. B Protein levels of FAM171B in the knockdown and overexpression cell lines. C Representative colony formation images in different groups. D Colony formation analysis of T24 and MB49 cells after knockdown and overexpression of FAM171B. E Cell viability of T24 and MB49 after knockdown and overexpression of FAM171B. F Representative transwell images of T24 and MB49 cells after knockdown and overexpression of FAM171B. G Statistical results of the number of invasive cells in each group of T24 and MB49 cells. H Representative images of migration in T24 and MB49 cells after knockdown and overexpression of FAM171B. I Statistical results of the number of migrated cells in each group of T24 and MB49 cells