Fig. 3
RNA sequencing of T24 cells to investigate the function of FAM171B. A Heatmap of the difference in overall mRNA expression between the FAM171B overexpression T24 cells (F1, F2 and F3) and the control T24 cells (N1, N2 and N3). The Pearson’s correlation coefficient was represented by a color scale. B Volcano plot of the differential gene expression between the FAM171B overexpression group and the control group. The vertical gray lines corresponded to two-fold up- and down-regulation, and the horizontal gray line represented a p value of 0.01. The red points represented significantly up-regulated gene, and the blue points represented significantly down-regulated gene. C Heatmap of specific differential genes between the FAM171B overexpression T24 cells and the control cells. D, E, F Histogram of GO annotations for the differential genes in biological processes (D), cellular components (E) and molecular functions (F). X axis represented the number of genes. Y axis showed the name of GO terms. G Histogram of KEGG analysis for the differential genes. X axis represented the number of genes. Y axis showed the name of enriched pathways. The p-value was represented by a color scale. The statistical significance increased from purple (relatively lower significance) to orange (relatively higher significance). H Histogram of eggNOG functional classification statistics for differentially expressed genes. X axis represented the function of the gene. Y axis showed the frequency (the number of genes)