Fig. 5

FAM171B stabilizes vimentin to promote the progression of bladder cancer. A Protein levels of FAM171B, vimentin, E-cadherin and N-cadherin in the FAM171B knockdown and overexpression T24 cell lines. B Protein levels of FAM171B and vimentin in the T24 cell lines in different groups. C Representative transwell images of T24 cells in different groups. D Statistical results of the number of invasive cells in each group of T24 cells. E Representative images of migration in T24 cells in different groups. F Statistical results of the number of migrated cells in each group of T24 cells. G Pictures of T24 cells in different groups. H Protein levels of FAM171B and vimentin in the T24 cells with or without FAM171B overexpression after treatmented with CHX (20 μg/ml) for various times. I Remaining vimentin protein level percentages in the T24 cells with or without FAM171B overexpression after treated with CHX for various times. J The western blot images showed the FAM171B regulated vimentin ubiquitination in T24 cells. Vimentin proteins were isolated from the FAM171B knockdown or overexpression T24 cells by Co-IP, and followed detected the ubiquitination of vimentin by western blot. input, whole cell lysate. K Representative IHC staining images of FAM171B and vimentin in FAM171B high or low expression group from tissue array. L Correlation analysis of IHC scores for FAM171B and vimentin from tissue array. M The pictures of tumors derived from human bladder cancer T24 cell line. N IHC staining pictures of FAM171B and vimentin expression and HE staining pictures in different groups. O The tumor volume in different groups. P The tumor weight in different groups. Q The average body weight of mice in different groups. R Analysis of FAM171B IHC score in tumors. S Analysis of vimentin IHC score in tumors