Fig. 6

FAM171B regulates CCL2 via HNRNPU and promotes TAM migration and infiltration in bladder cancer. A ELISA analysis of CCL2 secretion levels in MB49 cells with FAM171B overexpression or knockdown. B RIP results show that HNRNPU can immunoprecipitate with CCL2 precursor RNA. C qRT-PCR analysis of the relative RNA levels of precursor CCL2 and CCL2 in MB49 cells with HNRNPU knockdown or control. D ELISA analysis of CCL2 secretion levels in MB49 cells with HNRNPU knockdown or control. E Protein levels of FAM171B and HNRNPU in the MB49 cell lines in different groups. F ELISA analysis of CCL2 secretion levels in MB49 cells in different groups. G Analysis of migration of RAW264.7(mouse macrophages) toward MB49 cells in different groups. H Representative images of migration of RAW264.7(mouse macrophages) toward MB49 cells in co-culture chambers. I The pictures of tumors derived from mouse bladder cancer MB49 cell line. J IHC staining pictures of F4/80(macrophage marker) expression in different groups. K Representative images of flow cytometry used to evaluate F4/80 positive subpopulations. L The tumor weight in different groups. M The tumor volume in different groups. N The average body weight of mice in different groups. O Analysis of F4/80 positive population by IHC. P Analysis of F4/80 positive population by flow cytometry