Fig. 7

FAM171B regulates CCL2 expression to enhance TAM polarization towards the M2 phenotype. A Representative images of flow cytometry used to evaluate CD206 positive subpopulations in RAW264.7 co-cultured with MB49 cells in different groups. B Analysis of CD206 positive population by flow cytometry. C Representative images of immunostaining of Arg1 (M2 marker) in RAW264.7 co-cultured with MB49 cells. D Analysis of Arg1 positive population by immunostaining. E Analysis of EGF (M2 marker) secretion levels in RAW264.7 co-cultured with MB49 cells in different groups. F Analysis of IL-10 (M2 marker) secretion levels in RAW264.7 co-cultured with MB49 cells in different groups. G Representative images of flow cytometry used to evaluate CD206 and F4/80 positive subpopulations in MB49 subcutaneous graft tumor in different groups. H Analysis of CD206 and F4/80 positive population in MB49 subcutaneous graft tumor by flow cytometry. I Analysis of Arg1 and F4/80 positive population in MB49 subcutaneous graft tumor by immunostaining. J Representative images of immunostaining of Arg1 (M2 marker) and F4/80 (macrophage marker) in MB49 subcutaneous graft tumor in different groups