Fig. 2

INHBB stimulates GC cell proliferation, migration, and invasion in vitro. (A) Protein levels of INHBB was examined by Western blot in GES-1, MKN-7, MKN-45, HGC-27, MGC-803, NCI-N87 and Fu97 cells. (B) INHBB expression knockdown by shRNA in HGC-27 (upper) and MGC-803 (upper) and INHBB expression amplification in MKN-45 (lower left) and HGC-27 (lower right) as detected by Western blot. (C) Cell proliferation was verified by CCK-8 assay. The value of the absorbance (at 450 nm) was recorded from 0 to 96 h in HGC-27, MGC-803 and MKN-45 infected with shNC, shINHBB, Vector or INHBB amplified lentiviral virus (n = 3). (D) Cell apoptosis was detected by flow cytometry in HGC-27, MGC-803 and MKN-45 infected with shNC, shINHBB, Vector or INHBB amplified lentiviral virus (n = 3). (E) Cell migration and invasion were detected by transwell assay in HGC-27, MGC-803 and MKN-45 infected with shNC, shINHBB, Vector or INHBB amplified lentiviral virus (n = 3). (F) Stable knockdown or overexpression of INHBB affected protein levels of MMP2, Vimentin, Bcl2, Bax and Snail in GC cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001