Fig. 1From: TMEM147 aggravates the progression of HCC by modulating cholesterol homeostasis, suppressing ferroptosis, and promoting the M2 polarization of tumor-associated macrophagesTMEM147 was highly expressed in HCC and correlates with poor prognosis in HCC patients. (a) Differential expression of TMEM147 in the GDS4887 dataset. (b) TMEM147 expression levels in HCC (n = 369) and non-tumor tissues (n = 160) in GEPIA database. The differential analysis is based on the selected datasets with TCGA tumors versus TCGA normal + GTEx normal. Data are presented as mean ± SEM. *P < 0.01 (one-way ANOVA). (c) Representative images of TMEM147 IHC staining in normal liver tissues, high grade HCC and low-grade HCC tissues, and percentage of patients with HCC histological differentiation. Scale bars, × 100: 200 μm; × 400: 50 μm. (d) TMEM147 mRNA levels in HCC tissues and adjacent non-tumorous tissues. (e) qRT-PCR analysis of TMEM147 expression in HCC cell lines. (f) Western blot analysis of TMEM147 expression in HCC and matched non-tumor tissues. (g) TMEM147 protein levels in HCC cell lines and relative band density. (h) Kaplan–Meier overall survival and disease-free survival curves of patients with HCC with high (n = 182) and low (n = 182) expressions (stratified by median) of TMEM147 mRNAs in the GEPIA database using TCGA data (log-rank test). (i) Kaplan–Meier survival analysis of patients with HCC according to TMEM147 expression. All bar graphs are plotted as mean ± SEM of three independent experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001Back to article page