Fig. 2

TMEM147 promotes HCC proliferation, invasion, and migration in vitro and in vivo. (a) Growth curve assay based on CCK8 analysis in HCC cells. (b) Representative images of colony formation and statistical analysis of colony numbers. (c and d) Representative images of Transwell migration and Matrigel invasion assay for the indicated cells. Scale bars, 100 μm. (e) Representative images of subcutaneous xenograft derived from indicated HCC cells. (f) Representative images of lung metastasis specimens of lung metastasis derived from tail injection with indicated cells, TMEM147 overexpression formed more and larger metastatic nodules, whereas TMEM147 knockdown displayed fewer and smaller nodules. All experiments were performed three times and data are presented as mean ± SD. **p<0.01; ***p<0.001