Fig. 6

27HC enhances lipid metabolism in macrophages and activates M2 polarization. (a) Schematic of conditional medium. (b) Protein levels of M2 macrophage marker arginase 1 (ARG1) and CD206, FAO rate-limiting enzyme CPT1A and PPARγ in the indicated macrophages. (c) Cell immunofluorescence of ARG1 in the indicated macrophages. Scale bars: 50 μm. (d) Flow cytometry of CD163 + and HLA-DRα + macrophages in different groups. (e) Tumors were removed from mice and subjected to immunohistochemical staining with anti-ARG1 antibodies. Representative images are shown. Scale bars: 50 μm. (f) Lipid content of the indicated macrophages. Analyzed by ELISA assay. (g and h) Oxygen consumption rates (OCRs) of the indicated macrophages, and the statistic results depicting the basal and maximal respiration and spare respiratory capacity in the indicated macrophages by analyzing the OCRs. Statistical significance was determined by two-tailed Student’s t test between control MΦs and TAMs. *, P < 0.05; **, P < 0.01; ***, P < 0.001