Fig. 2

Expression of SF3B4 plasma protein and anti-SF3B4 autoantibodies as liquid biopsy biomarkers for HCC and their diagnostic performance in each cohort. A Positive dots of SF3B4 autoantibody expression from normal tissue and liver cirrhosis to HCC (Dx, HCC diagnosis). B ROC analysis of SF3B4 autoantibody. C Expression of plasma SF3B4 and expression of plasma anti-SF3B4 autoantibodies according to the stage of liver disease. Statistically significant differences were determined using one-way ANOVA with Tukey’s multiple comparisons test. Compared to HC; ***P <0.001, compared to LC; §§P <0.01. D AUCs of plasma SF3B4 in cohort 1 (left) and anti-SF3B4 autoantibody in cohort 2 (right) compared with serum AFP for diagnosing HCC. From left to right: diagnose all stages of HCC, mUICC stage I or II, and mUICC stage I