Fig. 2

M2-TAMs-secreted LINC01592 is transferred to esophageal cancer cells. A. The heatmap reflected one of the upregulated lncRNAs in the RNA sequence analysis of two pairs of M0-Exos and M2-Exos. B. TEM, NTA, and WB were adopted to measure the morphology, size, and surface markers of exosomes. Scale bar, 200 nm. C. Measuring the levels of LINC01592 in CM after M2-TAM treated with RNase A alone or RNase A and Triton X-100 simultaneously. D. Measuring the levels of LINC01592 in CM, soluble, and exosomes. E–F. RNA FISH in Eca-109 and a typical column chart. Scale bar, 50 µm. G. PKH26-labeled M2 exosomes were added to Phalloidin-labeled Eca-109 cells. Scale bar, 25 µm. The means ± SDs are provided (n = 3). **P < 0.01 and ***P < 0.001 according to two-tailed Student t-tests or one-way ANOVA followed by Dunnett tests for multiple comparisons