Fig. 3

M2-TAM-derived exosomal LINC01592 induces tumor immune escape. A. M2-Exos significantly inhibited T cell-mediated tumor cell killing compared to M0-Eoxs. However, after adding an exosome inhibitor Annexin V or knocking down LINC01592, the above phenomenon was restored B-E. Flow cytometry and real-time quantitative PCR results indicated that CD8⁺ T cells cocultured with M2-Exos-esophageal cancer cells showed lower proliferation and expression of IFN-γ, TNF-α, and Gzmb. However, after adding an exosome inhibitor Annexin V or knocking down LINC01592, the above phenomenon was restored. F-G. Typical animal in vivo imaging pictures, typical column charts, and CD8⁺ IHC pictures in different groups. Scale bar, 50 µm. The means ± SDs are provided (n = 3). **P < 0.01 and ***P < 0.001 according to two-tailed Student t-tests or one-way ANOVA followed by Dunnett tests for multiple comparisons. ns, no significant difference