Fig. 4

M2-Exos-derived LINC01592 mediates MHC-I expression by regulating the autophagy-lysosomal pathway. A. Different tumor cell lines were treated with M0-Exos and M2-Exos, and the expression level of MHC-I was detected by WB. The right side represents a typical column chart. B. Eca-109/PECC cells were treated with M0-Exos and M2-Exos, and the expression level of MHC-I was detected by WB using CHX assay. The right side represents a typical protein degradation curve. C. Eca-109/PECC cells with M0/M2-Exos simultaneously with the proteasome inhibitor MG132 and the lysosomal inhibitor Leupetin, and the expression level of MHC-I was detected by WB. D-E. Knockdown and overexpression of LINC01592 in Eca-109/PECC, respectively, and the expression level of MHC-I were detected by WB using CHX assay. The right side represents a typical protein degradation curve The means ± SDs are provided (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 according to two-tailed Student t-tests or one-way ANOVA followed by Dunnett tests for multiple comparisons. ns, no significant difference.