Fig. 6

LINC01592 promotes E2F6-mediated transcription of NBR1. A. We constructed the wild-type or mutant-type luciferase vectors based on the potential binding site of E2F6 to the NBR1 promoter. B. Luciferase activity was assayed in Eca-109/PECC cells transfected with luciferase vectors (wild type or mutant type) and, meantime, co-transfected with expression plasmids (empty vectors, E2F6 expression plasmids, or LINC01592 expression plasmids). C. ChIP, experiments of E2F6 (IgG as an internal control), were performed, and the co-precipitated DNA was subjected to PCR amplification with primers specific to the NBR1 promoter region. D. The level of NBR1 under ectopic expression of E2F6 or LINC01592 was detected by qRT-PCR and western blotting. The means ± SDs are provided (n = 3). **P < 0.01 and ***P < 0.001 according to two-tailed Student t-tests or one-way ANOVA followed by Dunnett tests for multiple comparisons. ns, no significant difference