Fig. 7

LINC01592 promotes esophageal cancer immune escape by regulating NBR1. A. LINC01592-OE/Eca-109/PECC cells significantly inhibited T cell-mediated tumor cell killing compared to Vector; however, knocking down NBR1 while overexpressing LINC01592 could rescue the above phenomenon. B-E. Flow cytometry and real-time quantitative PCR results indicated that CD8⁺ T cells cocultured with LINC01592-OE-esophageal cancer cells showed lower proliferation and expression of IFN-γ, TNF-α, and Gzmb; however, knocking down NBR1 while overexpressing LINC01592 could rescue the above phenomenon. F-G. Typical animal in vivo imaging pictures, typical column charts, and CD8⁺ IHC pictures in different groups. Scale bar, 50 µm. The means ± SDs are provided (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 according to two-tailed Student t-tests or one-way ANOVA followed by Dunnett tests for multiple comparisons. ns, no significant difference