Fig. 6

Effects of PLPP2 on the content of lipid rafts and cell proliferation. a A549 and NCI-H1299 cells were fixed after transfected with target siRNAs and IF was performed with antibodies recognizing flotillin-1 (red) and caveolin-1 (green). b-c Statistic analyses of flotillin-1 and caveolin-1 AOD values in A549 and NCI-H1299 cells after transfected with target siRNAs, n = 3. Bars, SD; **P < 0.01, ***P < 0.001, ****P < 0.0001; one way ANOVA. d-e Effects of PLPP2 knockdown on cell proliferation by CCK-8 in A549 and NCI-H1299 cells, n = 3. Bars, SD; *P < 0.05, ***P < 0.001, ****P < 0.0001; one way ANOVA. f-g Effects of PLPP2 knockdown on cell proliferation by colony formation assays in A549 and NCI-H1299 cells, n = 3. Bars, SD; ****P < 0.0001; one way ANOVA. h BEAS-2B and MLE-12 cells were fixed after transfected with target lentivirus particles and IF was performed with antibodies recognizing flotillin-1 (red) and caveolin-1 (green). i-j Statistic analyses of flotillin-1 and caveolin-1 AOD values in BEAS-2B and MLE-12 cells after transfected with target lentivirus particles, n = 3. Bars, SD; ns: no significance, **P < 0.01, ****P < 0.0001; one way ANOVA. k-l Effects of PLPP2 OE on cell proliferation by CCK-8 in BEAS-2B and MLE-12 cells, n = 3. Bars, SD; *P < 0.05, **P < 0.01; one way ANOVA