Fig. 1

CircRNA_0009092 was decreased in CRC tissues and was a stable circRNA in CRC cells.AHeatmap of differentially expressed circRNA based on RNA-seq analysis in 3paired CRC tissues. The red and blue strips represent high and low expression, respectively.BqRT-PCR assay showing the expression level of circ_0009092 in the tumor tissue and normal tissues of CRC (n= 80).CKaplan-Meier analysis of correlations between circ_0009092 expression levels and recurrence free survival (RFS) of 80 CRC patients.DKaplan-Meier analysis of correlations between circ_0009092 expression levels and overall survival (OS) of 80 CRC patients.ESchematic illustration showing the OGDH exon 19-20 circularization forming circ_0009092.FThe back-splice junction sequences of circ_0009092 were confirmed by Sanger sequencing.GStability of circ_0009092 and OGDH mRNA was assessed by RNase R treatment followed by qRT-PCR in HCT116 and Lovo cells.HThe expression levels of circ_0009092 in the nuclear and cytoplasmic fractions of HCT-116 and Lovo cells were assessed qRT-PCR.IFISH detection of circ_0009092 in HCT-116 and Lovo cells. Nuclei were stained with DAPI. Scale bar, 50 μm. Statistical analysis between two groups was performed using two-tailed t-test. One-way ANOVA statistical tests were adopted for more than two groups. Data are the means ± SD of three experiments. *P<0.05