Fig. 3

Circ_0009092 acted as the sponge of miR-665 in CRC cells.AqRT-PCR assay showing the expression levels of potential miRNA in the transfected CRC cells.BqRT-PCR analysis was performed to show the expression levels of miR-665 in 80 paired CRC tissues. (C) Spearman’s correlation analysis revealed a negative association between miR-665 expression and circ_0009092 expression in 80 CRC tissues.DKaplan–Meier plotter of the association of circ_0009092 with RFS in 80 CRC patients.EKaplan–Meier plotter of the association of circ_0009092 with OS in 80 CRC patients.FFISH analysis of the cellular colocalization of miR-665 and circ_0009092 in HCT-116 and Lovo cells. Scale bar, 20 μm.GSchematic representation of binding sequences between miR-665 and circ_0009092.HRIP assay was conducted for detecting the binding of circ_0009092 and miR-665 by using the Ago2 antibody.IThe luciferase activity of the WT luc-circ_0009092 or Mut luc-circ_0009092 after transfection with miR-665 mimic in CRC cells. Statistical analysis between two groups was performed using two-tailed t-test. One-way ANOVA statistical tests were adopted for more than two groups. Data are the means ± SD of three experiments. *P< 0.05, **P< 0.01