Fig. 6

Circ_0009092 regulated the recruitment of macrophages in CRC cells.ARepresentative immunofluorescence photographs for circ_0009092, CD68, DAPI in CRC samples. Bar = 50μm.BChemotaxis analysis of THP-1 macrophages toward supernatant from OE-circ_0009092, SH-circ_0009092-treated CRC cells.CChemotaxis analysis of THP-1 macrophages toward supernatant from OE-circ_0009092, SH-circ_0009092, miR-665 mimic and miR-665 inhibitor.DQuantification analysis of chemotactic cells in five fields was counted manually.EThe potential chemokines which promoted macrophage infiltration were identified by qRT-PCR in OE-circ_0009092, SH-circ_0009092-treated CRC cells.FELISA assay detected the CCL2 secretion level in OE-circ_0009092 or miR-665 mimic-treated CRC cells.GELISA assay detected the CCL2 secretion level in OE-NLK or SI-NLK-treated CRC cells.HChemotaxis analysis of THP-1 macrophages toward SH-circ_0009092 CRC cell supernatant with or without CCL2 Ab, miR-665 mimic CRC supernatant with or without CCL2 Ab.IQuantification analysis of chemotactic cells in five fields was counted manually. Statistical analysis between two groups was performed using two-tailed t-test. One-way ANOVA statistical tests were adopted for more than two groups. Data are the means ± SD of three experiments. *P< 0.05, **P< 0.01, ***P< 0.001