Fig. 8

Circ_0009092 inhibited the CRC tumor growth in vivo.AImages of xenograft tumors formed by HCT-116 cells stably expressing OE-circ_0009092, OE-NC and THP1 cells.BThe tumor weights of xenografts were evaluated.CThe tumor growth curves of the OE-circ_0009092 and the OE-NC group was shown.DqRT-PCR analysis of circ_0009092 expression in the OE-circ_0009092 and the OE-NC group.EqRT-PCR analysis of miR-665 expression in the OE-circ_0009092 and the OE-NC group.FqRT-PCR analysis of NLK expression in the OE-circ_0009092 and the OE-NC group.GWB analysis of E-cadherin, Vimentin, NLK, STAT3, p- STAT3, β-catenin, GSK3β, p-GSK3β, c-myc, and CCL2 in the OE-circ_0009092 and the OE-NC group.HFISH analysis of the cellular colocalization of miR-665 and circ_0009092 in the OE-circ_0009092 and the OE-NC group. Scale bar, 50 μm.Iimmunohistochemistry staining (IHC) and H&E staining of xenograft tumors.JFlow cytometry for analyzing the expression of M2 marker (CD163, CD206) and M1 marker (CD86) on macrophages in the tumor.n= 6/group. Statistical analysis between two groups was performed using two-tailed t-test. One-way ANOVA statistical tests were adopted for more than two groups. Data are the means ± SD of three experiments. *P< 0.05, **P< 0.01, ***P< 0.001. ns: no significance