Fig. 1

SNHG17 was overexpressed in TAMs from PDAC and associated with a poor prognosis in PDAC. A Schematic representation of the experimental workflow, including MACS (Magnet-Activated Cell Sorting) of TAMs and NTRMs from three PDAC patients and lncRNA sequencing of TAMs and NTRMs. B Volcano plot illustrating the differential expression level of lncRNAs between TAMs and NTRMs. C. The expression level of SNHG17 in tumor tissues (n = 179) and healthy tissues (n = 171) from the TCGA dataset. D-F Spearman correlation between SNHG17 and CD163 (D) or MRC1 (E) or IL10 (F) in PAAD from TCGA dataset. G The expression of SNHG17 in TAMs and NTRMs sorted from 30 PDAC patients. H Colocalization of SNHG17 (red) and CD163 or CD11b (green) in 96 clinical samples of PDAC as determined by fluorescence microscopy. DAPI staining (blue) displaying nuclei (DNA). Scar bar: up: 20 μm, down: 12 μm. I Intensity of immunofluorescence staining for SNHG17, CD163, and DAPI in tissue microarrays from 96 PDAC patients. Red represents SNHG17. Green represents CD163. Blue represents DAPI. J K-M survival curve of PDAC patients in the SNHG17 high expression and low expression groups in TAMs. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001