Fig. 6

Circ-hnRNPU inhibits protein glycosylation, growth, and invasion of gastric cancer cells by repressing NONO or c-Myc activity. a and b, Western blot assay showing the levels of O- and N-glycosylation in MKN-45 and AGS cells stably transfected with empty vector (mock), circ-hnRNPU, scramble shRNA (sh-Scb), or sh-circ-hnRNPU #1, and those co-transfected with NONO, c-Myc, sh-NONO #1, or sh-c-Myc #1. c and d, Representative images (left) and quantification (right) of soft agar (c) and matrigel invasion (d) assays indicating the anchorage-independent growth and invasion of MKN-45 and AGS cells stably transfected with mock, circ-hnRNPU, sh-Scb, or sh-circ-hnRNPU #1, and those co-transfected with NONO, c-Myc, sh-NONO #1, or sh-c-Myc #1. Student’s t test compared the difference in c and d. *P < 0.05. Data are shown as mean ± s.e.m. (error bars) or representative of three independent experiments in a-d