Fig. 1
Correlation of CAFs infiltration with clinical features and immunomodulation in bladder cancer. (A) Left: Immunohistochemistry of α-SMA in bladder cancer tissues and adjacent normal tissues. Scale bar = 50 μm. Right: Quantitative IHC analysis of α-SMA staining (n = 15). (B) Comparison of overall survival between high and low IHC score groups of α-SMA in bladder cancer tissues (n = 56). (C) Proportion of various stromal cells calculated by EPIC algorithm of TCGA data. (D) Comparison of overall survival between high and low CAFs proportion groups of TCGA data (n = 406). (E, F) Correlation of CAFs proportion with tumor grade and clinical stage of TCGA data. (G) Comparison of overall survival for five molecular subtypes of TCGA data (n = 406). (H) Comparison of CAFs proportion among five molecular subtypes. (I, J) GSEA and CIBERSORT immune infiltration analysis between high and low CAFs groups in TCGA. (K) IHC results showed that PDL1 was highly expressed in the region of high α-SMA expression. Scale bar = 50 μm. (L) Correlation of IHC scores of α-SMA with IHC scores of PDL1. * P < 0.05; ** P < 0.01; *** P < 0.001