Fig. 6
CXCL12 mediated the reduction of P62 ubiquitination. (A, B) The expression of P62 within 0–12 h after CXCL12 stimulation detected by qPCR in T24 and UMUC3 cells. (C, D) Western blotting showing the effect of MG132 (10µM, 6 h) and CXCL12 (50 ng/ml, 6 h) stimulation on P62 in T24 and UMUC3 cells. (E, F) Left: The degradation rate of P62 detected at the indicated time points by CHX chase assay (20µM) in T24 cells treated with MG132 (E) or CXCL12 (F) stimulation. Right: Quantitative curve of the level of remained P62. (G, H) Left: The degradation rate of P62 detected at the indicated time points by CHX chase assay (20µM) in UMUC3 cells treated with MG132 (G) or CXCL12 (H) stimulation. Right: Quantitative curve of the level of remained P62. (I, J) Western blotting of P62 ubiquitination stimulated by CXCL12 in T24 and UMUC3 cells