Fig. 2
Determination of hFAP density and binding assessment of αFAP TMs using flow cytometry. a the number of hFAP antigens per cell was determined for SCP-1 and HT1080 hFAP cell lines using QIFIKIT. b and c Titration curves for αFAP-scFv (b) and -IgG4 TM (c) binding to HT1080 hFAP and SCP-1 cells were obtained and apparent KD values were calculated. Data are plotted as MFI ± SD for three individual experiments