Fig. 3
Cytotoxicity assessment, EC50 values determination and profile of pro-inflammatory cytokines released by UniCAR T-cells redirected by αFAP TMs. a and b titration curves assessing the specific killing of UniCAR T-cells in the presence of αFAP-scFv (black) or -IgG4 (grey) TMs in combination with HT1080 hFAP (a) and SCP-1 (b) cells were determined using luciferase-based killing assays. Dose–response curves were plotted as mean specific lysis ± SD from three individual T-cell donors and half maximal effective concentration values (EC50) were accordingly determined. c and d co-cultures of UniCAR T-cells with monolayer HT1080 hFAP (c) and SCP-1 (d) cells in the absence (blue) or presence of αFAP-scFv (black) and –IgG4 (grey) TMs were incubated for 24 h followed by cytokine release assessment. Scatter bar plots represent the cytokine concentrations ± SD for four individual T-cell donors. Statistical significance was determined using one-way ANOVA with Bonferroni multiple-comparison test