Fig. 4
Microscopic image of a SCP-1 spheroid, cytotoxicity assessment and profile of pro-inflammatory cytokines released by UniCAR T-cells redirected by αFAP TMs to target FAP-expressing spheroids. a bright-field representative microscopic image of a SCP-1 spheroid obtained 48 h after cell seeding (left). Titration curves assessing the specific killing of SCP-1 spheroids by UniCAR T-cells in the presence of αFAP-scFv (black curve) or -IgG4 (grey curve) TMs in combination with SCP-1 spheroids were determined using luciferase-based killing assays. Dose–response curves and EC50 values were obtained and plotted as mean specific lysis ± SD from three individual T-cell donors. b co-cultures of UniCAR T-cells with spheroids of SCP-1 cells in the absence (blue) or presence of αFAP-scFv (black) and –IgG4 (grey) TMs were incubated for 24 h followed by cytokine release assessment. Scatter bar plots represent the cytokine concentrations ± SD for three individual T-cell donors. Statistical significance was determined using one-way ANOVA with Bonferroni multiple-comparison test