Fig. 5

Bright-field images and multiplex immunohistochemistry (mIHC) staining of SCP-1 spheroids co-cultured with UniCAR T-cells and αFAP TMs. a microscopic bright-field pictures of SCP-1 spheroids alone or co-cultured for 24 h with UniCAR T-cells, UniCAR T-cells and αFAP-scFv or -IgG4 TMs. b representative multicolor images with magnified views of SCP-1 spheroids co-cultured for 24 h with UniCAR T-cells, UniCAR T-cells and αFAP-scFv or -IgG4 TM stained for DAPI (blue), CD3 (green) and granzyme (Grz) B (magenta). Scale bars for images in panel b indicate 50 µm in the overview images and 10 µm in the zoom-in areas