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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization

Fig. 7

hnRNPA2B1 mediates the packaging of circATP9a2 into EVs. Notes: A qRT-PCR analysis of the expression level of EVs-circATP9A in BEAS-2B, A549, and H1299 cells; B The expression level of EVs-circATP9A in NSCLC cells after knockdown of circATP9A; C, D Transmission electron microscopy (TEM) and NanoSight were used to characterize the purified EVs from A549 cells; E Western blot analysis of EVs markers from A549 EVs or cell lysates; F qRT-PCR analysis of the expression level of circAtP9A in EVs from NSCLC cells treatment with GW4869 (an inhibitor of EVs secretion); G qRT-PCR analysis of the expression level of circATP9A in the CM of NSCLC cells after depletion of EVs by ultracentrifugation; H Western blot assay confirming the interaction between circATP9A and hnRNPA2B1 in circATP9A pull-down proteins; I RIP assay in A549 cells confirmed that circATP9A could be enriched by hnRNPA2B1; J Flow cytometric analysis of the expressions of CD206/HLA-DR in macrophages treated with different concentrations of exosomes isolated from supernatants of NSCLC cells. Numerical values denote the relative fluorescence intensity

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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