Fig. 1

Nutlin-3a reduces KRAS mutant NSCLC cell viability via the KRAS-PI3K/Akt pathways. A Cell viability was determined using MTT assay after nutlin-3a treatment for 48 h. B Cell proliferation examined using the colony formation assay after nutlin-3a treatment (2 μM) for 14 days (top); quantification of colony formation (bottom). C KRAS-GTP form detected by the KRAS activation assay after nutlin-3a treatment for 24 h. D KRAS protein stability was detected by western blotting after treatment of MG132 (10 μM) for 6 h before harvest. E KRAS downstream molecules were detected by western blotting. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control