Fig. 5

Downregulation of GFPT2 expression by nutlin-3a alters amounts of HBP metabolites in KRAS MT/p53 WT NSCLC cells. A, B Cells were treated with nutlin-3a (30 μM) for 24 h. Expression of GFPT2 mRNA was determined by RT-qPCR (A). Abundance of HBP metabolites analyzed by UHPLC–MS/MS (B). C Global O-GlcNAcylation of proteins and N-glycosylation with the terminal glycosyl/mannosyl binding lectin examined via western blotting. D Glycolysis was measured for 1 h by fluorescence emission. E The level of NADP + / NADPH ratio was measured as PPP flux. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control