Fig. 2

PHB2 promotes GC cell proliferation and tumorigenicity. A-D shRNA silencing of PHB2 (A) attenuated GC cell proliferation as shown in MTT assays (B), clonogenic assays (C) and 5-bromo-2′-deoxyuridine (BrdU) incorporation (D). Data are representatives or mean ± SEM; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparison. Scale bar, 1 cm. E, F PHB2 overexpression (E) promoted GC cell proliferation as shown in clonogenic assays (F). Data are representatives or mean ± SEM; n = 3 independent experiments, two-tailed Student’s t-test. Scale bar, 1 cm. G, H Overexpression of PHB2 (G) enhanced anchorage-independent growth of normal gastric epithelial cell line GES-1 (H). Data are representatives or mean ± SEM; n = 3 independent experiments, two-tailed Student’s t-test. Scale bar, 1 cm. I-K Representative photographs (I), tumour weight (J) and growth curves (K) of MKN-28.sh-scramble and MKN-28.sh-PHB2 xenografts in nu/nu mice. Data are representatives or mean ± SEM; n = 6 mice per group, two-tailed Student’s t-test (J), two-way ANOVA followed by Šídák’s multiple comparisons test (K). L Representative microscopic photographs (left panel), and quantitation of IHC staining for Ki67 (right panel) in FFPE sections of MKN-28.sh-scramble and MKN-28.sh-PHB2 xenografts. Data are representatives or mean ± SEM; n = 6 mice per group, two-tailed Student’s t-test. Scale bar, 20 μm. IRS: Immunoreactive score