Fig. 3

PHB2 binds to and co-localizes with SHIP2 in the cytoplasm of GC cells. A Endogenous PHB2 and SHIP2 were co-precipitated with each other in HGC-27 and MKN-28 cells. B Exogenous Flag-PHB2, and tGFP-SHIP2 were co-precipitated with each other in AGS cells. C Representative immunofluorescence photographs of PHB2 and SHIP2 co-localization in HGC-27 cells. Scale bar, 10 μm. D Western blotting showing subcellular localization of PHB2 and SHIP2. Cyt: cytoplasm; Nuc: nucleus. β-actin: Cyt marker; Histone H3: Nuc marker. E tGFP-SHIP2 was co-pulled down by recombinant GST-PHB1 or GST-PHB2, which was however diminished by PHB2 knockdown. Data are representatives of three independent experiments