Fig. 4

The SAM domain of SHIP2 and all regions of PHB2 except for the N-terminal are required for the interaction between PHB2 and SHIP2. A Schematic illustration of full-length SHIP2 (SHIP2-FL) and its corresponding truncates used in mapping experiments. B Deletion-mapping experiments showing that the SAM domain of SHIP2 is indispensable for its interaction with PHB2 in HEK293T cells. C Schematic illustration of full-length PHB2 (PHB2-FL) and the PHB2 mutants. D Deletion-mapping experiments showing that the N-terminal region of PHB2 is dispensable for its interaction with SHIP2 in HEK293T cells. Data are representatives of three independent experiments