Fig. 7

A Cross-reactive CD8 + T cells in pMHC tetramer staining. Unstimulated PBMCs from sample T-004 were incubated with pMHC tetramers loaded with TAA and MoAs homologous peptides. Dot plots show the reactivity against the SSX2 peptide (PE-A) and SSX2-BACT1 (APC-A), SSX2-BACT2 (PE-CF594-A) and SSX2-BACT3 (PE-Cy7-A). B Cross-reactive CD8⁺ T cells in pMHC tetramer staining after in vitro pre-immunization. Cross-reactivity against the paired SSX2-BACT2/BACT3 peptides was evaluated after an in vitro pre-immunization. PBMCs were stimulated with MoAs derived epitopes and tetramer staining was used to assess the cross-recognition of homologous TAA peptide