Fig. 4
From: PELI1: key players in the oncogenic characteristics of pancreatic Cancer
RPS3 Interaction with PELI1. A, B Proteins associated with PELI1 in PC PANC-1 cells overexpressing PELI1 by silver staining (A) and mass spectrometry analysis (B). C Endogenous immunoprecipitation verifying the binding of PELI1 to RPS3. D Schematic diagram of the deletion of different structural domains of PELI1, GST pull-down assay the inability of RPS3 to bind to PELI1 lacking the FHA structural domain. E Co-localization analysis of RPS3 and PELI1 in the cytoplasm using immunofluorescence. F, G qRT-PCR (F) and IHC (G) to detect the expression of RPS3 in PC and paracarcinoma tissues. H, I Immunohistochemistry showing a negative correlation between the expression of RPS3 and PELI1 protein levels. *P < 0.05, **P < 0.01