Fig. 5
From: PELI1: key players in the oncogenic characteristics of pancreatic Cancer
PELI1 Promotes RPS3 Degradation. A Western blot assay for the relationship between PELI1 and RPS3 expression in PC cells. B Increased RPS3 expression over time with increasing MG132 (20 μM) action time. C Decreased expression of exogenous RPS3 with increasing transfection of the Flag-PELI1 plasmid. D, E Prolongation of RPS3 protein half-life after MG132 (20 μM) treatment. F, G The degradation of RPS3 protein by PELI1 was inhibited after MG132 (20 μM) treatment. H, I PC cells transfected with Flag-PELI1, Myc-RPS3, and treated with CHX (20 μM) show increased RPS3 degradation in the PELI1 group. J RPS3 degradation slows down in the knockdown PELI1 group after CHX (20 μM) treatment. **P < 0.01