Fig. 1

Auranofin monotherapy. A Image-based quantification of organoid growth rate based on the Viability (V) metric, normalized to timepoint 0 (mean ± SD, n = 2). B Fitted dose–response curves of the mean (n = 2) Normalised Organoid Growth Rate (NOGR) metric for the treatment with Auranofin (500, 909, 1651, 3000 nM) for 120 h. C Normalized Area Over the Curve (AOC) values of the fitted NOGR dose–response curves following Auranofin treatment. D Representative images of organoids treated with vehicle (DMSO) or 909 nM Auranofin for 120 h. Magenta = label-free organoid segmentation by Orbits®; Green = raw cytotox green signal; LU_ = normal pulmonary organoids; NKI_ = lung cancer organoids; PDAC_ = pancreatic ductal adenocarcinoma organoids