Fig. 2

Predictive biomarkers for Auranofin response. A Percentile-based classification of Auranofin response into sensitive, intermediate and resistant groups. B Decision tree-based classification of the 3 response groups based on the topmost important features. PDAC_087 was excluded from this analysis to be used as a testing sample. Log2(counts per million reads, CPM) expression values for IGFL1 (C) and CA12 (D). E Partial correlation network for CA12. Grey edges correspond to positive correlation, red edges to negative correlation. The width of the edge is proportional to the absolute partial correlation value of the gene pair. F Positive and negative correlation of CA12 expression with members of the NF-kB signaling pathway. G Gene set enrichment analysis of NF-kB signaling related genesets, following differential expression analysis between the sensitive and resistant organoids. H Up- (red) and down- (blue) regulated genes of the NF-kB survival signaling pathway in the resistant versus sensitive organoids