Fig. 3

Trajectory analysis of tumor cells. a Trajectory analysis diagrams of pseudotime was shown. b The pseudotime in different samples. c The pseudotime in different states. d The top100 differential gene function enrichment heatmap. e The differential expression genes of different branches and GO BP pathways of different clusters were shown in heatmap. f The Dynamic changes of top5 differential expression genes determined cell fate between KO and WT group were shown. g Survival analysis of OSCC gene features using TCGA-PAAD based on Fig. 3f on the Gepia2 website (http:// gepia2. cancer- pku. cn/# index)