Fig. 4

Macrophage subpopulation analysis. a Subpopulation of macrophages were shown by t-SNE. b Fraction of cells in different clusters among six samples. c Number of cells in different clusters. d Number of genes in different clusters. e Number of UMIs in different clusters. f and g The expression level of Mrc1 in different subclusters of macrophage. h Proportion of M2 macrophages between KO and WT group in scRNA-seq results. i Immunofluorescence staining shows the expression of M2 macrophage between KO and WT group implanted with MOC-2 cells. j Immunohistochemistry demonstrated the expression of M2 macrophage between KO and WT group implanted with MOC-2 cells. k Immunofluorescence staining shows the expression of M2 macrophage between KO and WT group implanted with MOC-1 cells. l Immunohistochemistry demonstrated the expression of M2 macrophage between KO and WT group implanted with MOC-1 cells. m Flow cytometry analysis showed the expression levels of M2 macrophages between KO and WT group implanted with MOC-2 cells. n Flow cytometry analysis showed the expression levels of M2 macrophages between KO and WT group implanted with MOC-1 cells. (Student’s t-test, KO: n = 3, WT: n = 3, *P < 0.05.)