Fig. 2

MSLN promotes the migration and invasion of NSCLC cells in vitro. A Representative images and quantitative results of western blotting showing MSLN expression after transfection of PC9-BrM and PC9 cells with lentivirus. B The effect of MSLN expression on the migration capacity in PC9-BrM and PC9 cells assessed in a wound-healing assay (scale bar, 100 µm). C Transwell migration and invasion assays to determine the effect of altered MSLN expression on the migration and invasion of NSCLC cells (scale bar, 200 µm). D, E Western blot analysis of E-cadherin, N-cadherin, Slug and MMP7 expression in PC9-BrM cells and PC9 cells after alteration of MSLN expression. F-G The expression of MMP7 by PC9 cells and PC9-BrM cells was detected by qRT-PCR (F) and ELISA (G). (PC9-NC, PC9 cells transfected with negative control plasmid. PC9-OE, PC9 cells transfected with MSLN plasmid. PC9-BrM-NC, PC9-BrM cells transfected with negative control shRNA. PC9-BrM-SH1, PC9-BrM cells transfected with MSLN-targeted shRNA1. PC9-BrM-SH2, PC9-BrM cells transfected with MSLN-targeted shRNA2. Data are presented as mean ± SD, ns, no significance)