Fig. 3

MSLN promotes NSCLC cells penetration of endothelium by promoting cleavage of endothelial TJ proteins. A Schematic diagrams of a classic in vitro blood-brain barrier (BBB) model and the multi-organ microfluidic chip. B, C Representative images of the ability of tumor cells to penetrate the BBB in the Transwell assay and the chip (scale bar, 200 µm). D, E Representative confocal microscopy images showing the distribution of VE-cadherin, JAM-A and claudin-5 in a hBMVEC monolayer (scale bar, 20 µm). F, G Western blot analysis of VE-cadherin, JAM-A and claudin-5 expression in hBMVECs after treatment with conditioned medium from the indicated tumor cells. (TJ, tight junction. PC9-NC, PC9 cells transfected with negative control plasmid. PC9-OE, PC9 cells transfected with MSLN plasmid. PC9-BrM-NC, PC9-BrM cells transfected with negative control shRNA. PC9-BrM-SH1, PC9-BrM cells transfected with MSLN-targeted shRNA1. PC9-BrM-SH2, PC9-BrM cells transfected with MSLN-targeted shRNA2. Data are presented as mean ± SD, ns, no significance)