Fig. 5

MSLN regulates MET phosphorylation as well as protein and mRNA expression in NSCLC cells through the JNK signaling pathway. A Representative western blot images showing MSLN, p-JNK, t-JNK, p-MET and MET expression levels after MSLN knockdown in PC9-BrM cells. B, C PC9-BrM cells were treated with JNK-IN-8 and crizotinib at different concentrations of 0, 3, 6, and 12 µM for 24 h, and the expression of the indicated molecules was detected by western blotting. D-G PC9-BrM-NC cells, PC9-BrM-SH2 cells, PC9-NC cells and PC9-OE cells were incubated in serum-free medium for 24 h, and then PC9-BrM-NC cells and PC9-OE cells were treated with the designated inhibitors at 12 µM for 24 h. The expression of the indicated molecules was then detected by western blotting and qRT-PCR. (PC9-NC, PC9 cells transfected with negative control plasmid. PC9-OE, PC9 cells transfected with MSLN plasmid. PC9-BrM-NC, PC9-BrM cells transfected with negative control shRNA. PC9-BrM-SH1, PC9-BrM cells transfected with MSLN-targeted shRNA1. PC9-BrM-SH2, PC9-BrM cells transfected with MSLN-targeted shRNA2. Data are presented as mean ± SD, ns, no significance)