Fig. 6
From: Ropivacaine as a novel AKT1 specific inhibitor regulates the stemness of breast cancer
Higher GGT1 correlates to shorter overall survival of breast cancer patients. A mRNA level of GGT1 in 1113 breast cancer tissues and 113 benign normal tissues from TCGA database. B IHC analysis of GGT1 expression in breast cancer (Tumor) and peritumor tissues (Normal) from our cohort (n = 70), (scale bar = 100 μm). C Protein level of GGT1 in 4 breast cancer tissues and their adjacent normal tissues was determined by western blot. D Protein level of GGT1 in six breast cancer cell lines (MCF-7, MDA-MB-231, SKBR-3, T47D, BT-549 and SUM149) and a non-transformed mammary epithelial cell line (HMEC-hTERT) was determined by western blot. E Protein level of GGT1 in mammosphere derived from MCF-7 and MDA-MB-231 cells and their parental cells was evaluated by western blot. F Kaplan–Meier analysis of the relationship between GGT1 expression levels and overall survival of breast cancer patients from TCGA database. *p < 0.05; **p < 0.01; ***p < 0.001 (log-rank test in F, others unpaired two-tailed Student’s t test)