Fig. 7

Effects of GGT1 on CSC-like phenotypes of MCF-7 cells in vitro. A-B RNA (A) and protein (B) level of GGT1 in MCF-7 cells stably transfected with shRNAs against GGT1 (shGGT1-1 and shGGT1-2) or negative control (shCtrl) were determined by qRT-PCR and western blot, respectively. C Stem cell markers (CD133, OCT4, SOX2) in GGT1-depleting MCF-7 cells were analyzed by western blot. D CD44⁺/CD24.⁻ subpopulation in GGT1-depleting MCF-7 cells was measured by FACS analysis. E Mammosphere formation of GGT1-depleting MCF-7 cells (scale bar = 100 μm). F Migration and invasion of GGT1-depleting MCF-7 cells were examined by transwell assays (scale bar = 100 μm). G Cell viability of GGT1-depleting MCF-7/ADR cells exposed to the indicated concentration of ADR. H Cell apoptosis of GGT1-depleting MCF-7/ADR cells exposed to ADR (10 μM) were assessed by FACS analysis. Results are shown are shown as mean ± S.D from at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 (Two-way ANOVA test in G, others unpaired two-tailed Student’s t test)