Fig. 9

GGT1 enhances activation of NF-κB signaling pathway. A RNA-seq data of MCF-7-shCtrl or -shGGT1 cells. The volcano map showing differentially-expressed genes. B KEGG analysis of the top 10 enriched pathways in MCF-7-shCtrl or -shGGT1 cells from RNA-seq data. C Heatmap showing differentially expressed genes in NF-κB signaling pathway in MCF-7-shCtrl or -shGGT1 cells. D GSEA analysis of the enrichment of NF-κB signaling pathway in MCF-7-shCtrl or -shGGT1 cells from RNA-seq data. E NF-κB transcriptional activity in MCF-7 and MDA-MB-231 cells stably transfected with shRNAs against GGT1 (shGGT1-1 and shGGT1-2) or negative control (shCtrl) was determined by NF-κB activation reporter assay. F NF-κB transcriptional activity in MCF-7 and MDA-MB-231 cells transfected with GGT1 overexpression plasmid (GGT1-OE) or empty plasmid (Vector) was determined by NF-κB activation reporter assay. G Protein levels of GGT1, p-iκBa, iκBa, p-NF-κB and NF-κB in GGT1-depleting and GGT1-overexpressing MCF-7 and MDA-MB-231 cells were determined by western blot. Results are shown are shown as mean ± S.D from at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 (unpaired two-tailed Student’s t test)