Fig. 3

Identification of Breg-related genes. (A) We compiled immunosuppressive gene sets and Breg cell marker genes. (B) Differentially-expressed genes between IL10- and IL10 + B cells were analyzed. (C) A total of 27 genes were identified as Breg-related genes, which were found to overlap. (D) Functional enrichment analysis was conducted to understand the biological roles of the 27 genes. (E) A PPI network was constructed to reveal the internal interactions among the 27 genes. (F) The expressions of the 27 Breg-related genes were used to compute the ssGSEA scores for 326 B cells extracted from the scRNAseqDB. (G) The levels of IL10 were compared between the high- and low-ssGSEA score subgroups. (H) Positive associations were observed between the ssGSEA score and the regulation of IL10 production. (I) Negative associations were found between the ssGSEA score and the regulation of immune system processes. ssGSEA, single-sample Gene Set Enrichment Analysis; PPI, protein-protein interaction; *P < 0.05; **P < 0.01; ***P < 0.001